[Histonet] Immunofluorescence in exfoliated cells from the cervix uterine

[tiang yeepeng]

Dear all,
I am currently staining for the E6 protein in exfoliated cells obtained from the uterine cervix by indirect immunofluorescence method. However, the noise was found to be terrible due to the presence of dirts and other particles eg: bacterias commonly found in the cervix and also the actinomycetes. Repeated washings with PBS were not able to rid those particles. Can anyone please advise? Thanks!

With regards,
TIANG YEE PENG
Department of Medical Microbiology,
Faculty of Medicine,
University of Malaya.
 
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