[Histonet] neutrophils

[Scott A. Ely]

Dorothy,
Because neutropils contain myeloperoxidase, it you don't quench the tissue with h2o2 (as is done in IHC), they 
will turn DAB brown.  This is a pretty good and simple way of detecting neutropils.  I don't have a protocol off the 
top of my head, but if you do what is otherwise an IHC stain, only skip the h2o2 and don't use any antibody, all the 
neutrophils will be brown; other cells don't generally have any type of peroxidase.  Also, at least in humans, CD15 
is pretty neutropil specific and a good marker---if you want to go the IHC route.

Scott Ely, MD MPH
Section of Hematopathology
Department of Pathology
Weill Medical College of Cornell University
New York Presbyterian Hospital
525 E. 68th Street
New York, NY 10021
PH:  212-746-2442

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----- Original Message -----
From: histonet-request <@t> lists.utsouthwestern.edu
Date: Friday, June 9, 2006 1:00 pm
Subject: Histonet Digest, Vol 31, Issue 14