[Histonet] whole eyes

[Favara, Cynthia (NIH/NIAID) [E]]

Patsy,

I have been doing a fair number of eyes lately but in paraffin. I find
that for optimal fixation it is helpful to inject the eye with fixative
shortly after the necropsy.

c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: Patsy Ruegg [mailto:pruegg <@t> ihctech.net] 
Sent: Friday, June 09, 2006 9:11 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] whole eyes

Dear Eye People,
It has been about 25 years since I processed eyes.  I just got some
fresh
whole rabbit eyes that I want to fix and process into GMA.  They came in
a
media with antibiotic in it one wet ice.  I assume that I put them whole
into cold formalin to maintain the shape, then at some point I will need
to
extract the fluid inside without collapsing so that I can process into
GMA.
I am going to use graded changes of the GMA as my dehydrating agent
avoiding
alcohol (this will be necessary for the final product which will contain
hydrogel implant which does not survive solvent processing, thus the
choice
of GMA embedding).  I seem to remember using a needle and syringe to
extract
some fluid and then replace it with the GMA in stages????
Thank you so much for your advise,
Patsy
 
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net <http://www.ihctech.net/> 
 

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