[Histonet] neutrophils

Webb, Dorothy L Dorothy.L.Webb <@t> HealthPartners.Com
Fri Jun 9 07:58:25 CDT 2006

We are staining some research tissue (rabbitt?) for neutrophils using
the chloroacetate esterase kit from Sigma with an inflamed appendix for
a control.  The control turned out beautiful, but, nothing is showing up
in the research tissue.  We did not  gross in the research tissue, only
processed it according to our routine processing schedule.  According to
the "fellow" who is doing this project, the formalin was expired on the
research tissue.  Does anyone have any ideas on this case??  I am at a
loss and the pathologist helping the "fellow" to read these tissues out
is wanting me to come up with a solution, of course!!  So, HELP and
thanks, as always!!
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