[Histonet] mounting very thick specimen

[Elizabeth Chlipala]

We routinely embed whole rat corneas.  We use the smallest coverslip
possible and place a good bit of mounting media on the slide and then
place the cornea on the slide into the mounting media, from there we put
a bit more mounting media on top of the specimen and then coverslip.  We
use a carrage bolt (3/8 x 4) with 3 hex nuts 3/8-16 that we place on top
of the coverslip to get the specimen to remain flat when drying, it
looks funny and you need to be careful when you place the bolt on, but
it does work.  We needed to do this because the cornea is not completely
flat and even though it was notched a bit of weight on the coverglass
helped the specimen remain flat.  You might not need the bolts, if your
specimen is nice and flat, you just need the right amount of mounting
media.  The amount that you need is going to be dependent upon the
mounting media that you use, since no mounting media is the same and the
amount of resin in the media is going to be different, you just have to
work out the number of drops of media that you will need so you don't
get any air bubbles once the media drys.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Sebastien Thuault
Sent: Wednesday, June 07, 2006 4:37 PM
To: Histonet
Subject: [Histonet] mounting very thick specimen


Does anyone have tips to mount very thick specimen (400 um) on slides in
aqueous or resinous medium?

Thanks!

Sebastien Thuault, PhD
Center for Neurobiology and Behavior
Columbia University
PI Annex/Kolb Institute
1051 Riverside Drive
New York, NY 10032

Phone: +1-212-543-5037
Fax: +1-212-795-7997


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