[Histonet] Overnight HIER

[Orr, Rebecca]

Mark,

I would imagine a lower temp would work, I haven't tried that. I have
had great success with running overnight in a 60'C environment.  The
Biocare pressure cookers can be programmed to run at a stable temp
overnight.
The staining is generally very good and the morphology is just perfect
with a citrate solution.
Morphology isn't as good with overnight using a high pH.

Hope this helps.

Becky Orr CLA,HT(ASCP)
Assistant Manager, Anatomic Pathology
Evanston Northwestern Healthcare
847-570-2771