[Histonet] overnight HIER

Sebree Linda A. la.sebree <@t> hosp.wisc.edu
Tue Jun 6 14:26:54 CDT 2006


In one of the workshops I attended in Toronto ( I wish I could remember
the name of the presenter), they mentioned doing this but not at 60
degrees C but a lower temp. like 37 or 45.  Hopefully this person will
see your inquiry and respond.  I believe they did this for finicky
antigens and tissues that were prone to come off the slides.

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
FAX: (608)262-7174

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mark
Sent: Tuesday, June 06, 2006 2:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] overnight HIER

Hi everyone.  In a meeting this morning someone mentionned that they had
been toold to do an overnight HIER treatment.  They had never heard of
this and wanted to know more. Is anyone doing this?  Is it simply
heating the slides at say 60C for 12-18 hours in a water bath or is
there more to it-what buffer, what temp??  Any information would be
greatly appreciated.
Mark Elliott
Vancouver BC

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