[Histonet] bone decalcification

Kemlo Rogerson kemlo.rogerson <@t> waht.swest.nhs.uk
Tue Jun 6 02:11:52 CDT 2006

Make thin slices of bone and suspend in 10% soln of EDTA in 0.1M Tris buffer
pH 6.95 (add KOH pellets to achieve that). Keep soln in cold room (4 degrees
C) and stir with magnetic stirrer. Add bone which ought to decalcify within
24 hours. Freeze slices mount on chuck and cut sections; the knife may have
to be cooled with a block of CO2 ice or to cool the microtome.

Courtesy of the great God R.D. Lillie.

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
  Not all that is gold glitters, not all who wander are lost. --Tolkein 

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