[Histonet] bone decalcification
Kemlo Rogerson
kemlo.rogerson <@t> waht.swest.nhs.uk
Tue Jun 6 02:11:52 CDT 2006
Make thin slices of bone and suspend in 10% soln of EDTA in 0.1M Tris buffer
pH 6.95 (add KOH pellets to achieve that). Keep soln in cold room (4 degrees
C) and stir with magnetic stirrer. Add bone which ought to decalcify within
24 hours. Freeze slices mount on chuck and cut sections; the knife may have
to be cooled with a block of CO2 ice or to cool the microtome.
Courtesy of the great God R.D. Lillie.
Kemlo Rogerson
Pathology Manager
Ext 3311
DD 01934 647057
Mob 07749 754194
Not all that is gold glitters, not all who wander are lost. --Tolkein
This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on its
contents: to do so is strictly prohibited and may be unlawful. Please inform
me that this message has gone astray before deleting it. Thank you for your
co-operation
More information about the Histonet
mailing list