[Histonet] Anti roll plate on IEC plus cryotome, need help

[Rittman, Barry R]

I have not used an IEC for some time but there were always problems with
the anti roll plate.
We ended up cutting glass ones of our own (from a 3 x 2" glass slide),
adding a thin strip of sellotape along each side to provide clearance
for the section and then spraying the surface with a Teflon spray to
prevent sections sticking. These plates were significantly better than
the plastic anti roll plates.
We never had much luck getting good serial sections of skin using the
brush technique but as Gayle says whatever works for you.
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle
Callis
Sent: Monday, June 05, 2006 4:39 PM
To: Ze Lu; Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Anti roll plate on IEC plus cryotome, need help

The antiroll plate for this old IEC was probably developed for use with
c 
profile steel knives.

I suggest you take the plate off and learn to do frozen sections using a

sable brush, or some type of brush that works best for you. A very nice 
tutorial on how to use brush technic is found at
www.pathologyinnovations.com

Talk to a technical services person at Triangle Biomedical Systems (?) 
(TBS) about a different or workable antiroll plate or before you spend
the 
money on a device that will be useless. TBS has a website and they sell
IEC's.

Personally, we prefer no antiroll plate and do brush technic for 99% of
our 
cryostat work  - it is faster, no readjustment of plates, etc.

At 02:02 PM 6/5/2006, you wrote:
>Hi, histonet friends,
>
>We have an old IEC plus cryotome. We changed to a disposable blade
system. 
>According to the IEC plus mannual, we adjust the anti roll plate.
However, 
>we tried couple sectioning, the section always curved and get folded. I

>used different crytome, for all of them, there is only small angle (or 
>space) between blade holder and anti roll plate where secion go
through, 
>that section can not be folded after leave the block. However for IEC 
>plus, it really has big angle according to their description. I think 
>someone know what I am talking about if he used this model. Can someone

>share his experience on this? Also, we are going to change the anti
roll 
>plate, can someone kindly let us know which product will fit to this 
>model. We appreciate very much.
>
>
>
>=====================
>Ze Lu, Ph.D.,
>Optimum Therapeutics, LLC
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)



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