[Histonet] (no subject)

Kellar, Eric C Eric.C.Kellar <@t> questdiagnostics.com
Mon Jun 5 09:58:09 CDT 2006


I agree with Joyce! Your infiltrating paraffins could also be contaminated with a carry over of xylene during processing. If your final paraffins are not changed frequently enough, too much residual xylene deposits into the paraffins (you can usually smell it). This can result in sinkholes and an uneven block face, as the xylene evaporates out.

Eric C. Kellar
Quest Diagnostics, Inc

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Weems,
Sent: Monday, June 05, 2006 9:58 AM
To: Timo Väisänen; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)

This sounds like your tissue is not well dehydrated and cleared. When those steps are inadequate, the paraffin cannot impregnate the tissue adequately. The alcohol and xylene then evaporate, leaving the sinkhole in the tissue. 

My 2 cents worth, j:>)
Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Timo
Sent: Monday, June 05, 2006 9:54 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Dear all,

Has anyone encountered the following problem? The surface of newly embedded
paraffin blocks start to become concave only after few days. This is not a
problem with normal tissue specimens but with for example small biopsies the
uneveness creates difficulties. Biopsies on the edge of the block run out if
you try to trim the block enough to get the samples in the middle of the block
to the same section with the other samples. I quess paraffin can contract but I
have always thought that this takes years. Could there be something wrong with
the paraffin? Any suggestions?   
Thank's in advance,


Timo Väisänen PhD 
Oulu University Hospital
Department of Pathology

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc.

The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited.

More information about the Histonet mailing list