[Histonet] RE: Histonet Digest, Vol 31, Issue 5

[Langxingpan]

Dear Tiang Yee,

You can coat your slides using the following method.

Slide Coating with 3 - aminopropyltriethoxysilane (APES; Sigma A-3648)
1.	Soak glass slides in acetone containing 2% APES for 1 to 5 mins;
2.			Wash the slides in acetone or distilled water
briefly;
3.		Dry the slides at 42 °C.

All the best,


Langxing Pan, M.D., Ph.D.
Pantomics, Inc.
457 Mariposa Street
San Francisco, CA 94107
Direct: 1-415-863-2380
langxingpan <@t> pantomics.com
www.pantomics.com 

Message: 2
Date: Sun, 4 Jun 2006 15:47:07 +0000
From: "tiang yeepeng" <yeepengtiang <@t> hotmail.com>
Subject: Re: [Histonet] section adhesion on slides
To: <histonet <@t> lists.utsouthwestern.edu>, <mcauliff <@t> umdnj.edu>
Message-ID: <BAY108-W89CBD65E8611269556E75DF970 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

I coat my slides with poly-L-lysine but it seemed that I lost many cells
during the washing steps in IHC. I use a centrifuge my cells onto the slides
using the cytospin. Any idea or comment, Geoff?

With regards,
TIANG YEE PENG
Department of Medical Microbiology,
Faculty of Medicine,
University of Malaya.