[Histonet] re: GAD vs. paraformaldehyde

Carl Hobbs carl.hobbs <@t> kcl.ac.uk
Thu Jun 1 14:14:19 CDT 2006

Without going into the debate, may I offer two example pics of Chemicon's
GAD 65/67 Ab for you to look at? Here: http://www.immunoportal.com/index.php
(in the Immunohisto picture gallery)
It seems, to me, to work very well in pwax sections of rat brain, after
HIER; brain was immersion - fixed in 4% Formalin in PBS pH7.3( made using
the commercially available 37% Formalin solution). It works equally well in
PFA-derived fixing fluid, made to the same recipe, whether immersion -fixed
or perfusion -fixed, IMHO.
Best wishes

More information about the Histonet mailing list