[Histonet] Attention: Joe Galbraith - Tau AT8
Cynthia A Delong
DELONG_CYNTHIA_A <@t> LILLY.COM
Thu Jun 1 07:02:11 CDT 2006
This message is to Joe Galbraith- I was searching the Histonet archives
and I ran across his protocol for Tau AT8 from endogen.
It said "1:2000 dil, no pretreatment, 15-15 dakoautostainer"
I was needing more details. I have been working with the biotinylated
MN1020B and also I tried the MN1020.
Joe or anyone else who has gotten this antibody to work in paraffin 8 um
sections please send me the protocol.
I tried the biotinylated in HRP.
Antigen retrieval in both citrate and formic acid
3% Hydrogen Peroxide in PBS 5 min, Power Block 5 min
dilutions ranging from 100-2000, for 2 hrs and ON
Vector ABC 30 min - 1 hr, DAB 5- 10 min.
I just received the MN1020
and tried it no pretreament, 3% Hydrogen peroxide 5 min, Power Block 5
min, 1:2000 antibody 15 minutes, 1:500- Southern Biotech goat anti mouse
kappa FITC- 30 min.
Cynthia A DeLong
LRL - Corporate Center
DC0533 - 48B/3042
355 E Merrill St
Indianapolis, IN 46225
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