[Histonet] Problems with eyes

[Cheryl Crowder]

Hello - I am working with whole eye retinas.  The retinas are collected, 
defatted, rehydrated to distilled water, stained, dehydrated through graded 
alcohols and cleared in xylene.  Here's the problem:  When the tissues hit 
the xylene they disintegrate!  Not wash off - I mean disintegrate.  Have any 
of you heard of this problem before and, if so, what did you do about it?   
I would appreciate any comments, thoughts or help with this problem.  Maybe 
one of you who works exclusively with eyes has seen this before.  Thank you 
for your help,
Cheryl


Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Anatomic Pathology
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Skip Bertman Drive
Baton Rouge, LA  70803

225-578-9734
FAX:  225-578-9720