[Histonet] Bone Marrow Iron Stains

Rene J Buesa rjbuesa <@t> yahoo.com
Wed Jul 12 15:37:10 CDT 2006


Kelly:
  Try decalcifying with EDTA which is a chelating agent used at pH7; not like an acid decalcifyer. It always worked fine for me.
  Hope this will help you!
  René J.

Kelly Larson <kfineout <@t> hotmail.com> wrote:
  
I am having a problem getting my bone marrow aspirate cell blocks and
core biopsies to stain positively for Iron. The aspirate smears are
staining fine. The cell block and core biopsy are fixed in 10%NBF and
the core biopsy is decaled for one hour in Cal-Ex (Fisher). Any
suggestions??

Kelly ([1]kfineout <@t> hotmail.com)

Pathology Services of West MI

References

1. mailto:kfineout <@t> hotmail.com
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