[Histonet] Re: biomaterials

[Linda Jenkins]

Annaclaudia,

You stated:

Hi, I'm working with a scaffold made of polylactic acid and I' cutting frozen
sections of the material embedded in tissuetek to be staine with H/O. The
sections do not adhere at all on normal slides or on polylysine slides. Can
anyone suggest me what kind of slide to use?
thanks
annaclaudia

Dept of Publ, Prev and Clin Medicine
Human Anatomy Unit
Second University of Naples

         Try formalin gelatin or Elmer's glue recipe.  Polylysine does not 
mix well with polylactic acid and acts as a rinse agent!
         I will be glad to send you procedure if you can't find it in a 
histology text.
Thanks,
Linda


Linda Jenkins, HT
Clemson University
Dept. of Bioengineering
Clemson, SC 29634-0905
864.656.5553
http://www.ces.clemson.edu/bio/research/histo.htm