[Histonet] Trichrome following Histofix?

Monfils, Paul PMonfils <@t> Lifespan.org
Mon Jul 10 14:23:05 CDT 2006


I have received some blocks of Histofix-fixed liver, with a request for
trichrome staining.  The trichromes (Masson's or Gomori's) are coming out
with excessive blue staining.  The collagen is a nice clear blue, but
everything that should be red is a dense purple.  Has anyone else
experienced this problem with Histofix?  Any way around it?



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