[Histonet] Re: Histonet Digest, Vol 32, Issue 10

Chinmay Chitnis chitnis.c <@t> neu.edu
Mon Jul 10 13:12:59 CDT 2006


hi
   Can anyone suggest me a source for MMP-1 for bovine collagen?
Thanks
Chinmay


histonet-request <@t> lists.utsouthwestern.edu wrote:


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>When replying, please edit your Subject line so it is more specific
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>
>Today's Topics:
>
>   1. gastric cancer (Heike Grabsch)
>   2. Shirley is away : 10 July 2006 (Monday) (Shirley PHUA)
>   3. Cryostat disinfection (donna rossi)
>   4. Re: Re: Giemsa Stain (gillian.2.brown <@t> gsk.com)
>   5. RE: Histonet Digest, Vol 32, Issue 9 (Sanders, Julie, VHACIN)
>   6. Re: Cryostat disinfection (Rene J Buesa)
>   7. Re: Histonet Digest, Vol 32, Issue 8 (JOHN P COLEMAN)
>   8. CA IX (Fatma Bahar SUNAY)
>   9. RE: Cryostat disinfection (Anne Van Binsbergen)
>  10. RE: Re: Histonet Digest, Vol 32, Issue 8 (Dawson, Glen)
>  11. RE:decontamination. (Charles  Scouten)
>  12. Re: IHC workload (Brian Chelack)
>  13. Troubleshoot on mounting tissue (Judy Choi)
>
>
>----------------------------------------------------------------------
>
>Message: 1
>Date: Sun, 9 Jul 2006 18:10:12 +0100
>From: "Heike Grabsch" <H.I.Grabsch <@t> leeds.ac.uk>
>Subject: [Histonet] gastric cancer
>To: <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
>	<C6AC304539F43C4196253FD94B7960F32514E0 <@t> HERMES2.ds.leeds.ac.uk>
>Content-Type: text/plain; charset="iso-8859-1"
>
>Why do you want to do a special test for gastric cancer? If you want to 
diagnose it on a resection speciment, you don't need one. But may be 
you have a particular question in mind, such as idnetifying diffuse 
type gastric cancer cells in a biopsy or soemthing like that or do you 
want to know the CK combination for the differential diagnosis? If you 
are more specific, I may be able to help
>Heike 
> 
>Dr Heike Grabsch, MRCPath MD
>Gastrointestinal Cancer Research Group
>The Leeds Institute of Molecular Medicine
>Section of Pathology and Tumour Biology
>Wellcome Trust Brenner Bldg, Level 4
>St James's University Hospital 
>Beckett Street
>Leeds
>LS9 7TF
>England
> 
> 
>
>________________________________
>
>From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of 
histonet-request <@t> lists.utsouthwestern.edu
>Sent: Sun 09/07/2006 18:07
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: Histonet Digest, Vol 32, Issue 9
>
>
>
>Send Histonet mailing list submissions to
>        histonet <@t> lists.utsouthwestern.edu
>
>To subscribe or unsubscribe via the World Wide Web, visit
>        http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>or, via email, send a message with subject or body 'help' to
>        histonet-request <@t> lists.utsouthwestern.edu
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>When replying, please edit your Subject line so it is more specific
>than "Re: Contents of Histonet digest..."
>
>
>Today's Topics:
>
>   1. Re: Mordant for PTAH (RSRICHMOND <@t> aol.com)
>   2. Special stains and IHC for Gastric Cancer (Diana Schleicher)
>   3. Re: IHC Workload (Alan Bishop)
>   4. RE: IHC workload (Orr, Rebecca)
>
>
>----------------------------------------------------------------------
>
>Message: 1
>Date: Sat, 8 Jul 2006 14:10:50 EDT
>From: RSRICHMOND <@t> aol.com
>Subject: [Histonet] Re: Mordant for PTAH
>To: histonet <@t> lists.utsouthwestern.edu
>Message-ID: <431.473e7b6.31e14f2a <@t> aol.com>
>Content-Type: text/plain; charset="ISO-8859-1"
>
>Charlene Henry HT (ASCP), QIHC at St. Jude Children's Research Hospital
>[Memphis, Tennessee] asks:
>>>We are trying to rid the lab of all mercury products and I was wondering
>what everyone is using as a mordant for the PTAH. We are currently 
using 4%
>mercuric chloride and I would like to replace it with another 
mordant.<< and Rene
>J Buesa replies>>Try using 5% potassium dichromate at 60ºC for 30 
minutes. It
>has worked for me.<<
>
>But then you have to dispose of chromium, which is as difficult as 
mercury to
>get hauled away, I think.
>
>But what does anyone use phosphotungstic acid hematoxylin (PTAH) for 
nowadays
>anyway? Forty years ago it was of some use for staining muscle 
striations (in
>suspected rhabdomyosarcomas) and astrocytes - if the tissue had been 
fixed in
>Zenker/Helly (mercury, chromium, and formaldehyde). I recall it being 
said
>that the chief virtue of PTAH was that it took overnight to do the 
stain, and
>that gave you time enough to try to identify an unusual tumor. (It also 
took
>three months to make PTAH - you didn't add an oxidant, just put it on 
the back
>porch like sun tea.)
>
>According to Giuseppe Verdi or his librettist, Aida praised it to the 
skies
>("Omnipotente Ptah" - sorry about that), but I think PTAH's fortunes 
have been
>in decline ever since.
>
>Bob Richmond
>Knoxville TN and Gastonia NC
>
>
>------------------------------
>
>Message: 2
>Date: Sat, 8 Jul 2006 12:30:14 -0700 (PDT)
>From: Diana Schleicher <dianaschleicher <@t> yahoo.com>
>Subject: [Histonet] Special stains and IHC for Gastric Cancer
>To: histonet <@t> lists.utsouthwestern.edu
>Message-ID: <20060708193014.94230.qmail <@t> web53503.mail.yahoo.com>
>Content-Type: text/plain; charset=iso-8859-1
>
>I am currently a histotech student. I am writing a paper and preparing 
a power point presentation on the subject of Gastric Cancer.  I would 
like to find out what tests labs are performing for this disease.  
Thank you.
>
>Diana
>
>                       
>---------------------------------
>Sneak preview the  all-new Yahoo.com. It's not radically different. 
Just radically better.
>
>------------------------------
>
>Message: 3
>Date: Sun, 9 Jul 2006 11:43:31 +1200
>From: "Alan Bishop" <muddymoo <@t> gmail.com>
>Subject: Re: [Histonet] IHC Workload
>To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
>Cc: histonet <@t> lists.utsouthwestern.edu
>Message-ID:
>        <b474df0d0607081643n3dda13b1nc107a444bba9a465 <@t> mail.gmail.com>
>Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
>Some interesting stats there.
>
>Our lab does around 20,000 IHC slides a year. Most blocks already 
processed
>and embedded from us doing the routine histology but the daily workload 
of
>recutting, adding controls and the whole IHC process is done by one 
person!
>All of our workload is done by hand at the moment and all achieved in 
less
>than a standard working day - usually all issued to the paths by mid
>afternoon.
>
>>From the survey I should probably think about getting some more staff 
for
>the IHC :-)
>
>Cheers
>
>Alan
>
>On 08/07/06, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:
>>
>> Jennifer:
>>   From a survey I finished in Feb./06 (Advance Magazine 3 July/06) I 
can
>> give you some general information:
>>   1-the average of IHC tests/year in 22 foreign labs = 18,000
>>   2-the average for USA labs (23) = 8,000 [General averages between 300
>> and 69,000 slides/year for all labs).
>>   3- the difference in IHC workload between foreign and USA labs is
>> significant (P<0.05)
>>   4- usually the slides are cut in the same lab (average 2 hours/day).
>>   5-46% of labs use autostainers (different makes).
>>   6-for total workload = between 1 and 27 HTs (Average = 8; data from 
122
>> labs).
>>   7-lab assistants: between 0 and 7, average = 2 (48 labs).
>>   Hope this will help you!
>>   René J.
>>
>>
>> Jennifer MacDonald <JMacDonald <@t> mtsac.edu> wrote:
>>   I have a few questions for IHC labs related to workload and staffing.
>> Thank you.
>> 1. How many slides per day?
>> 2. Are slides processed, embedded, and cut by the IHC staff or 
elsewhere?
>> 3. Automation or manual.
>> If automation what instrument?
>> 4. Number of staff members to perform the workload?
>> How many histotechs? How many lab assistants?
>>
>> Thanks to all who help with this.
>> Jennifer MacDonald
>> Mt. San Antonio College
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>>
>>
>> ---------------------------------
>> Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls.  Great
>> rates starting at 1¢/min.
>> _______________________________________________
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>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>------------------------------
>
>Message: 4
>Date: Sun, 9 Jul 2006 10:09:15 -0500
>From: "Orr, Rebecca" <ROrr <@t> enh.org>
>Subject: [Histonet] RE: IHC workload
>To: <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
>        <A7FC3A4F98964A44B0B71B7B25EA6F960760F659 <@t> EXCHANGE03.enhnet.org>
>Content-Type: text/plain;       charset="us-ascii"
>
>Hi Jennifer,
>Our IHC lab is separate from the Routine Histology lab. We do not rotate
>techs into the IHC lab here.  We have a Technical Specialist (HTL, QIHC)
>who is responsible for the IHC lab.  He works 7:30am-4pm. Then we have a
>Tech (HT,) who comes in from 10:30am -7:00pm (she actually LIKES THAT
>SHIFT!) So we have 2 people covering almost 12hours.
>This helps when the Docs come in late in the afternoon with IHC
>requests; we have coverage until long after they are gone to cut
>everything that comes in. Overnight in the oven is a luxury we love
>having. On occasion though, we do run slides the same day.
>
>Lately we've been looking at the LEAN 6-sigma set up where instead of
>one large batch of slides once a day, we're processing several smaller
>runs to cut down on total run processing time.  I'd be glad to discuss
>this further in a separate email, if you're interested.
>We run approx. 100-150 slides/day with a Ventana Benchmark and a Biocare
>Nemesis.  The Autostainer platform of the Nemesis is great for working
>up research projects that require larger numbers of slides.
>
>To finish answering your questions, the Histology lab cuts the H/E
>slides.
>Docs read the H/E and order IHC.  We get the orders and then hunt down
>the blocks (some Docs are trained to submit the blocks) and cut our own
>IHC.  We have a cassette holder re-alignment instrument that keeps all
>of the microtomes lined up, so blocks can be cut from any microtome.
>There are always cases that arise where an FNA core biopsy is submitted
>and the IHC lab cuts the H/E and takes unstained slides immediately.
>IHC lab does not embed.
>I recommend that the person leading the IHC section have a propensity
>for running these stains.  It would be advantageous for this person to
>have a keen interest in keeping the lab updated with new antibodies.
>
>It kind of depends,  on your Pathologists.  Our lab is part of a
>teaching hospital with 13 Pathologists and a dozen Pathology residents
>each doing separate research projects.  We are always working on a
>poster or abstract for one of them.  So in our IHC lab, we need a
>progressive leader who is interested in working with the consistency of
>change...someone who has the experience and the progressive attitude to
>research new antibodies and juggle research and clinical assignments on
>a very regular basis.
>
>There are many labs that  require  a menu of 20-30 antibodies to be run
>in a consistent routine, so in this case the IHC personnel requirements
>might be a bit  different.(My opinion)
>
>Hope this helps,
>Becky
>
>Becky Orr CLA,HT(ASCP)QIHC
>Assistant Manager, Anatomic Pathology
>Evanston Northwestern Healthcare
>847-570-2771
>
>
>Message: 15
>Date: Thu, 6 Jul 2006 14:43:18 -0700
>From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
>Subject: [Histonet] IHC Workload
>To: histonet <@t> lists.utsouthwestern.edu
>Message-ID:
>       
><OF431E4EEF.873CB8D4-ON882571A3.00759DF2-882571A3.007759C5 <@t> mtsac.edu>
>Content-Type: text/plain; charset="US-ASCII"
>
>I have a few questions for IHC labs related to workload and staffing.
>Thank you.
>1.  How many slides per day?
>2.  Are slides processed, embedded, and cut by the IHC staff or
>elsewhere?
>3.  Automation or manual.
>        If automation what instrument?
>4.  Number of staff members to perform the workload?
>        How many histotechs?  How many lab assistants?
>
>Thanks to all who help with this.
>Jennifer MacDonald
>Mt. San Antonio College
>
>------------------------------
>
>
>
>
>
>------------------------------
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>End of Histonet Digest, Vol 32, Issue 9
>***************************************
>
>
>
>------------------------------
>
>Message: 2
>Date: Mon, 10 Jul 2006 02:10:28 +0800
>From: Shirley PHUA <Shirley_PHUA <@t> hsa.gov.sg>
>Subject: [Histonet] Shirley is away : 10 July 2006 (Monday)
>To: histonet <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
>	
<OF0CACF283.D70C9BC7-ON482571A6.0063D612-482571A6.0063D613 <@t> gems2.gov.sg>
>	
>Content-Type: text/plain; charset=US-ASCII
>
>I will be out of the office from  10/07/2006 to 10/07/2006.
>
> I will return on 11 July 2006 (Tuesday).
>
>Pathologists : I will process your requests when I return. Otherwise, if
>urgent, please contact Henry.
>
>
>
>
>------------------------------
>
>Message: 3
>Date: Sun, 09 Jul 2006 19:42:18 -0400
>From: "donna rossi" <djmr55 <@t> hotmail.com>
>Subject: [Histonet] Cryostat disinfection
>To: histonet <@t> lists.utsouthwestern.edu
>Message-ID: <BAY103-F93FA40FCCF6D0FA1EABC2DE6A0 <@t> phx.gbl>
>Content-Type: text/plain; format="flowed"
>
>
>   Here  we  go  again!   According to CAP we are to be shutting down 
the
>   cryostat  and  disinfecting  it every week for cryostats that are 
used
>   daily. How  often  should it be shut down if it is used perhaps once 
a
>   week?   Do  you determine this by number of frozens being done or 
type
>   of  tissue being  cut  or  just  pick  a  time frame?   We are a 
small
>   hospital and this will impact our work flow but if CAP says it must 
be
>   so thennnnnnnnnnnnnn. Your help is greatly appreciated.
>                                                                  Donna 
,
>    Sharon Reg Health System
>
>
>------------------------------
>
>Message: 4
>Date: Mon, 10 Jul 2006 08:52:43 +0100
>From: gillian.2.brown <@t> gsk.com
>Subject: Re: [Histonet] Re: Giemsa Stain
>To: RSRICHMOND <@t> aol.com
>Cc: histonet <@t> lists.utsouthwestern.edu,
>	histonet-bounces <@t> lists.utsouthwestern.edu
>Message-ID:
>	<OFA160D9A7.EB7D6D8A-ON802571A7.002AD60D-802571A7.002B266C <@t> gsk.com>
>Content-Type: text/plain; charset=us-ascii
>
>Hi,
>Guess no one told me how hard it is as I do make my own.  We do have 
very 
>ancient stocks so maybe I wont be able to once the components have gone.
>
>
>Regards
>
>Gill Brown
>
>
>
>
>
>
>RSRICHMOND <@t> aol.com 
>Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
>07-Jul-2006 19:08
> 
>To
>histonet <@t> lists.utsouthwestern.edu
>cc
>
>Subject
>[Histonet] Re: Giemsa Stain
>
>
>
>
>
>
>Kim Tournear asks >>I'm in need of a recipe for a giemsa stain using 
the 
>powder. I also need a recipe for making light green from powder.<<
>
>I've never - in more than 40 years - encountered anyone - including an 
>intensely giemsophilic research lab 40 years ago - Wolbach's 
colophonium 
>rosin and 
>all - who made up their own Giemsa stain solution from the dry powder. 
>It's 
>supposed to be extremely difficult to do. Scrupulous exclusion of water 
is 
>
>essential - that applies to stock bottles of the ready-made solution 
also.
>
>Kemlo has given you adequate advice about Light Green SF. - And yes, 
>Kemlo, 
>many labs on this side of the pond have no books. American science 
>education 
>marches on into the new century.
>
>Bob Richmond
>Knoxville TN and Gastonia NC
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
>
>------------------------------
>
>Message: 5
>Date: Mon, 10 Jul 2006 07:36:30 -0400
>From: "Sanders, Julie, VHACIN" <Julie.Sanders <@t> va.gov>
>Subject: [Histonet] RE: Histonet Digest, Vol 32, Issue 9
>To: <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
>	<2D4ACE41DEFE93428F23D77988EFBCB50E601F <@t> VHAV10MSGA2.v10.med.va.gov>
>Content-Type: text/plain;	charset="us-ascii"
>
> 
>
>I have a few questions for IHC labs related to workload and staffing. 
>Thank you.
>1.  How many slides per day?  
>2.  Are slides processed, embedded, and cut by the IHC staff or
>elsewhere?
>3.  Automation or manual.
>        If automation what instrument?
>4.  Number of staff members to perform the workload?
>        How many histotechs?  How many lab assistants?
>
>Thanks to all who help with this.
>Jennifer MacDonald
>Mt. San Antonio College
>
>Jennifer, at the VAMC Cincinnati we run between 10-30 slides per day for
>IHC.  The blocks are embedded and cut by the Histology staff.  We have a
>Ventana Benchmark which gives us the option of running overnight stains
>since we only have one shift, this works great for us.  There's only 3
>of us, myself and 2 techs, so we all perform IHC.
>Our TAT is usually 24 hours or less, normally the docs get their IHC's
>on the same day ordered if they order them before 12 noon.  If it is a
>longer procedure (TTF-1, etc.) we run these overnight.
>Hope this helps.
>
>Julie 
>
>
>------------------------------
>
>Message: 6
>Date: Mon, 10 Jul 2006 05:45:01 -0700 (PDT)
>From: Rene J Buesa <rjbuesa <@t> yahoo.com>
>Subject: Re: [Histonet] Cryostat disinfection
>To: donna rossi <djmr55 <@t> hotmail.com>,
>	histonet <@t> lists.utsouthwestern.edu
>Message-ID: <20060710124501.443.qmail <@t> web61212.mail.yahoo.com>
>Content-Type: text/plain; charset=iso-8859-1
>
>Donna:
>  Regardless of the amount of frozen cases, the fundamental criteria is 
the type of specimen.
>  Do you cryosection TB cases or any which are a potential 
contamination source? If so you should disinfect the cryostat after 
each one of those cases.
>  CAP recommends shutting down and cleaning weekly because the 
probability of having those types of cases on a weekly basis.
>  Being a small facility I think that you should prepare a schedule 
based on usage of the cryostat or even better, ask CAP if they have 
some "latitude" regarding small facilities.
>  CAP is always willing to answer.
>  I hope this will help.
>  René J.
>
>donna rossi <djmr55 <@t> hotmail.com> wrote:
>  
>Here we go again! According to CAP we are to be shutting down the
>cryostat and disinfecting it every week for cryostats that are used
>daily. How often should it be shut down if it is used perhaps once a
>week? Do you determine this by number of frozens being done or type
>of tissue being cut or just pick a time frame? We are a small
>hospital and this will impact our work flow but if CAP says it must be
>so thennnnnnnnnnnnnn. Your help is greatly appreciated.
>Donna ,
>Sharon Reg Health System
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> 		
>---------------------------------
>Why keep checking for Mail? The all-new Yahoo! Mail Beta shows you when 
there are new messages.
>
>------------------------------
>
>Message: 7
>Date: Mon, 10 Jul 2006 08:50:14 -0400
>From: "JOHN P COLEMAN" <JPCOLEMA <@t> sentara.com>
>Subject: [Histonet] Re: Histonet Digest, Vol 32, Issue 8
>To: <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
>	<mailman.0.1152550800.10937.histonet <@t> lists.utsouthwestern.edu>
>Content-Type: text/plain; charset=US-ASCII
>
>PTAH mordant- we use bouin's and it gives good results.
> 
>IHC staffing/workload.
>I am the Senior tech for a local reference lab doing IHC for 8
>hospitals, our load is about 500 slides per day. They are cut by the IHC
>staff (me and one other tech). We have 5 Biogenex i6000's, we also do
>manual IF, Muscle biopsy histochemical stains and Bone Marrow
>histochemical stains. Short answer- 500 slides a day, 2 techs, no
>ancillary support, 5 biogenex i6000s
> 
>John P.J. Coleman HT(ASCP)QIHC
>Interim Technical Clinical Specialist
>Anatomic Pathology
>Sentara Laboratory Services
>Cell/Voicemail(757)335-2159
>pager: (757)456-6695
>
>
>------------------------------
>
>Message: 8
>Date: Mon, 10 Jul 2006 13:15:45 +0000
>From: "Fatma Bahar SUNAY" <sunay <@t> balikesir.edu.tr>
>Subject: [Histonet] CA IX
>To: histonet <@t> lists.utsouthwestern.edu
>Message-ID: <20060710131216.M89620 <@t> balikesir.edu.tr>
>Content-Type: text/plain;	charset=iso-8859-9
>
>
>Hi everybody,
>
>I am looking for a reliable protocol for IHC staining of CA IX in HeLa 
cell 
>cultures. Thanks a lot.
>
>Dr. Bahar Sunay
>Balikesir University
>Turkey 
>--
>Open WebMail Project (http://openwebmail.org)
>
>
>
>
>------------------------------
>
>Message: 9
>Date: Mon, 10 Jul 2006 17:21:53 +0400
>From: "Anne Van Binsbergen" <vanann702 <@t> skmc.gov.ae>
>Subject: RE: [Histonet] Cryostat disinfection
>To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>, "donna rossi"
>	<djmr55 <@t> hotmail.com>,	<histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
>	<E384C7149472254BAA70D4BE8F1EA61202190C3C <@t> SKMCMAIL.skmc.gov.ae>
>Content-Type: text/plain;	charset="iso-8859-1"
>
>Surely universal precautions apply - all specimens should be treated 
the same - fresh specimens are all potentially hazardous. 
>Common sense should prevail. 
>
>In an HIV/TB infested country - where I come from - we had 'panic 
stations' if we knew the status of the patient(HIV/TB +ve), and 
everyone donned as much protective gear as they could find!!! - but 
everyone seemed to forget the first few frozens of the day, where we 
did not know the HIV/TB status - did that make those specimens less 
infectious/dangerous??? NO!!!
>
>If you have only one cryostat and have frequent frozens you need to 
take a slightly different approach - educate your surgeons and 
pathologists - do imprints and smears where possible - switching off, 
defrosting and cleaning a cryostat will cripple this sort of lab.
>In my current lab I have 3 cryostats and few frozens and (lucky for me) 
have relatively 'safe' patients, so I can afford to defrost on a 
rotational basis. Not everyone is as fortunate. 
>Just my opinion
>Annieinarabia
> 
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J 
Buesa
>Sent: Monday, July 10, 2006 4:45 PM
>To: donna rossi; histonet <@t> lists.utsouthwestern.edu
>Subject: Re: [Histonet] Cryostat disinfection
>
>Donna:
>  Regardless of the amount of frozen cases, the fundamental criteria is 
the type of specimen.
>  Do you cryosection TB cases or any which are a potential 
contamination source? If so you should disinfect the cryostat after 
each one of those cases.
>  CAP recommends shutting down and cleaning weekly because the 
probability of having those types of cases on a weekly basis.
>  Being a small facility I think that you should prepare a schedule 
based on usage of the cryostat or even better, ask CAP if they have 
some "latitude" regarding small facilities.
>  CAP is always willing to answer.
>  I hope this will help.
>  René J.
>
>donna rossi <djmr55 <@t> hotmail.com> wrote:
>  
>Here we go again! According to CAP we are to be shutting down the
>cryostat and disinfecting it every week for cryostats that are used
>daily. How often should it be shut down if it is used perhaps once a
>week? Do you determine this by number of frozens being done or type
>of tissue being cut or just pick a time frame? We are a small
>hospital and this will impact our work flow but if CAP says it must be
>so thennnnnnnnnnnnnn. Your help is greatly appreciated.
>Donna ,
>Sharon Reg Health System
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>Histonet mailing list
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>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> 		
>---------------------------------
>Why keep checking for Mail? The all-new Yahoo! Mail Beta shows you when 
there are new messages.
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>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>------------------------------
>
>Message: 10
>Date: Mon, 10 Jul 2006 08:23:38 -0500
>From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
>Subject: RE: [Histonet] Re: Histonet Digest, Vol 32, Issue 8
>To: "JOHN P COLEMAN" <JPCOLEMA <@t> sentara.com>,
>	<histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
>	<B3D65550856D0146B900D401EE313D4B2D0A0F <@t> dynams.dynacaremilwaukee.com>
>Content-Type: text/plain;	charset="iso-8859-1"
>
>John,
>
>All I can say is...WOW.  That is an UNREAL amount of work output.  I 
hope you are paid handsomely for this almost superhuman effort.
>
>Well Done,
>
>Glen Dawson
>IHC Manager
>Milwaukee, WI
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of JOHN P
>COLEMAN
>Sent: Monday, July 10, 2006 6:50 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Re: Histonet Digest, Vol 32, Issue 8
>
>
>PTAH mordant- we use bouin's and it gives good results.
> 
>IHC staffing/workload.
>I am the Senior tech for a local reference lab doing IHC for 8
>hospitals, our load is about 500 slides per day. They are cut by the IHC
>staff (me and one other tech). We have 5 Biogenex i6000's, we also do
>manual IF, Muscle biopsy histochemical stains and Bone Marrow
>histochemical stains. Short answer- 500 slides a day, 2 techs, no
>ancillary support, 5 biogenex i6000s
> 
>John P.J. Coleman HT(ASCP)QIHC
>Interim Technical Clinical Specialist
>Anatomic Pathology
>Sentara Laboratory Services
>Cell/Voicemail(757)335-2159
>pager: (757)456-6695
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>------------------------------
>
>Message: 11
>Date: Mon, 10 Jul 2006 09:07:33 -0500
>From: "Charles  Scouten" <cwscouten <@t> myneurolab.com>
>Subject: [Histonet] RE:decontamination.
>To: <djmr55 <@t> hotmail.com>,	"HISTONET"
>	<histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
>	
<5784D843593D874C93E9BADCB87342AB01307224 <@t> tpiserver03.Coretech-holdings.
com>
>	
>Content-Type: text/plain;	charset="us-ascii"
>
>I don't have an answer, but will post this to the histonet.  You should
>consider subscribing.  I assume you are not presently in the market for
>a self decontamininating crystat that cycle itself every night and be
>ready in the morning?
>
>
>Cordially,
>Charles W.  Scouten, Ph.D. 
>myNeuroLab.com 
>5918 Evergreen Blvd. 
>St. Louis, MO 63134 
>Ph: 314 522 0300 x 342
>FAX  314 522 0377 
>cwscouten <@t> myneurolab.com 
>http://www.myneurolab.com 
> 
>
>-----Original Message-----
>From: djmr55 <@t> hotmail.com [mailto:djmr55 <@t> hotmail.com] 
>Sent: Saturday, July 08, 2006 1:57 PM
>To: Charles Scouten
>Cc: Doug Martin; Drew N. Mehta; Charles Scouten
>Subject: myNeuroLab Question
>
>Subject:Cryostat Decontamination
>
>Question:I work in a small hospital histology lab. Until recently we
>used chlorhexidine digluconate in 70% ETOh to decontaminate our cryostat
>at -14C.  CAP now says we need to disinfect with 70% and also
>decontaminate with a tuberculocidal at an interval appropriate to our
>facility.  How do you determine what is appropriate?  Number of routine
>cases cut on a cryostat?  We have never had anything that is suspected
>of T.B., or HIV being cut on our cryostat. Is decontaminating 1 every 3
>months okay or once a year?  We need to shut this thing down over a
>weekend and that is another probelem with OR scheduling and no backup
>instrument .  HElP!!  Donna Rossi (ASCP) Histosupervisor Sharon Regional
>Health System
>
>
>
>------------------------------
>
>Message: 12
>Date: Mon, 10 Jul 2006 09:08:26 -0600
>From: Brian Chelack <brian.chelack <@t> usask.ca>
>Subject: [Histonet] Re: IHC workload
>To: histonet <@t> lists.utsouthwestern.edu
>Message-ID: <002a01c6a432$b23d9c20$0f13e980 <@t> PDS04>
>Content-Type: text/plain; charset=us-ascii
>
>Some great information here, but I am wondering if you have any data
>pertaining to the number of slides stained per FTE (full time 
equivalent)
>per year. Another bit of information that would be useful is scope of
>staining performed by the average lab; that is, how many different 
stains
>are in their repertoire? The workload in a lab that runs 50,000 of the 
same
>stain is entirely different from a lab that runs 500 slides of each of 
100
>different stains. Finally, how responsible are the techs for 
examination of
>the slides. Do they simply run the stains and ship everything out for 
the
>pathologists, do they examine the controls only, or do they run an 
initial
>visual screening of the cases? Each of these options changes the 
workload
>for the techs and the pathologists dramatically.
>
> 
>
>Thanks,
>
> 
>
>Brian Chelack
>
>Prairie Diagnostic Services
>
>52 Campus Drive
>
>Saskatoon, SK
>
>S7N 5B4
>
> 
>
>306-966-7241
>
> 
>
>
>
>------------------------------
>
>Message: 13
>Date: Mon, 10 Jul 2006 11:52:09 -0400
>From: "Judy Choi" <clarinjc <@t> hotmail.com>
>Subject: [Histonet] Troubleshoot on mounting tissue
>To: histonet <@t> lists.utsouthwestern.edu
>Message-ID: <BAY106-F3088888B905277C0F313AFDD6B0 <@t> phx.gbl>
>Content-Type: text/plain; format=flowed
>
>Hi,
>
>This is my first time using this listserve, so I hope this works.
>
>I have a question about mounting tissues (PFA perfused rat brain 
tissues to 
>be specific).  I processed them for IHC using ABC complex system and 
DAB and 
>used deionized water as my mounting medium.  However, when I put the 
tissue 
>in water, it swells tremendously and easily becomes wrinkled.  I think 
as a 
>consequence of the swelling, there are holes throughout my tissues.  (I 
>didn't use hydrogen peroxide until DAB, and it was at an extremely low 
>concentration of less than 0.1%.)
>
>Anyways, is my hypothesis plausible (that mounting my tissue in DI 
water can 
>cause tissues to swell and create holes)?  If so, does anyone know how 
I can 
>eliminate this problem?  Or can it be another factor (bad 
cryoprotectant, 
>bad perfusion, bad buffer)?  I appreciate any feedback I can get.
>
>Thank you!
>Judy
>
>
>
>
>
>------------------------------
>
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>End of Histonet Digest, Vol 32, Issue 10
>****************************************
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