[Histonet] michaels fixative

[Chris Pomajzl]

First of all, it is not really a fixative. It is more of a static transport
media that minimizes proteolysis. Although, it does "fix" immunoglobulins in
the tissue, which is needed for Immunofluorescence.

I cannot provide a reference, but I have read that normal saline works as
well, if not better, than Michel's sloution.

Michel's is good for long-term storage (2-3 days), whereas saline is good
for very short periods of time.

----- Original Message ----- 
From: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, July 07, 2006 10:57 AM
Subject: [Histonet] michaels fixative


Can anyone tell me something about this fixative?

Thanks!



Betsy Molinari HT (ASCP)

Texas Heart Institute

Cardiovascular Pathology

6770 Bertner Ave.

Houston,TX 77030

832-355-6524

832-355-6812 (fax)



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