[Histonet] Detection of phospholipidosis -reg
PKamalavenkatesh <@t> wockhardtin.com
PKamalavenkatesh <@t> wockhardtin.com
Tue Jul 4 03:24:09 CDT 2006
Dear Histonetters,
This is with regard to detection of
phospholipidosis in rodent tissue sections. I was told to develop a rapid,
sensitive screening method for detection of phospholipidosis in rodent
tissues. The chemicals I am dealing with are known for inducing
phospholipidosis in liver, spleen, kidney, adrenals and eye. In the
literature, it was recommended that ultra structural examination of the
sections is the gold standard for detection of phospholipidosis (presence
of lamellar bodies in lysosomes). Histologically, the only changes observed
are the vacuolations in the respective tissues. Also acid phosphatase and
nile red staining are recommended as marker of lysosomal storage disorders
and histochemical staining of phospholipids respectively. Now my question
is whether any of our members are having any sort of experience to deal
with this since doing Electron Microscopy for regular screening will be
very difficult. Hence, my request is anybody can give me a sound advice to
recommend a procedure for rapid and sensitive detection of phospholipidosis
in rodents.
Thanks and regards
REGARDS
Dr.P.Kamalavenkatesh
New Drug Discovery- Biology
Wockhardt Research Center
D-4, MIDC, Chikalthana
Aurangabad. Maharastra, India
E.mail : Pkamalavenkatesh <@t> wockhardtin.com
Please Visit our New Corporate Web Site www.wockhardt.com
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