[Histonet] Re: Histonet Digest, Vol 26, Issue 14

[Gloria Limetti]

RE;  Eosin too pink!

We love the pink! but just dilute it woth more 95% or
stain for less time.

Gloria Limetti
University of Pittsburgh/Vestibular Research

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>    1. RE: Eosin too pink
>       (Marshall Terry Dr,	Consultant
> Histopathologist)
> 
> 
>
----------------------------------------------------------------------
> 
> Message: 1
> Date: Fri, 13 Jan 2006 17:54:18 -0000
> From: "Marshall Terry Dr,	Consultant
> Histopathologist"
> 	<Terry.Marshall <@t> rothgen.nhs.uk>
> Subject: RE: [Histonet] Eosin too pink
> To: "Parker, Helayne" <HParker <@t> Skaggs.Net>,
> 	<histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> 
>
<FE2DB935F8BBB546B8A1BBF3459C5A1F05F54912 <@t> LIL.xRothGen.nhs.uk>
> Content-Type: text/plain;	charset="iso-8859-1"
> 
> Wouldn't we all *love* to see just what is
> considered too pink:-)
> An old pathologist friend of mine used to put a
> pinch of bicarb in the eosin when the techs were not
> looking.
> He had a thing of not liking it too pink. For me, I
> can't have it too pink, as long as the nuclei are
> blue.
> 
> Dr Terry L Marshall, B.A.(Law),
> M.B.,Ch.B.,F.R.C.Path
>  Consultant Pathologist
>  Rotherham General Hospital
>  South Yorkshire
>  England
>         terry.marshall <@t> rothgen.nhs.uk
> 
> -----Original Message-----
> From: Parker, Helayne [mailto:HParker <@t> Skaggs.Net]
> Sent: 13 January 2006 16:42
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Eosin too pink
> 
> 
>  Hi List,
>   We are currently doing our H & E stain by hand and
> using the
> Richard-Allan 7211 Hematoxylin and their Eosin Y. 
> Our pathologist is
> still complaining of slides being TOO PINK.  I have
> cut the eosin step
> down to just 5 dips from 15 dips, and still too
> pink.  The Eosin is very
> rich. 
> 
> This is our protocol:
> Hydrate to water like normal (thru Clear Rite 3,
> 100% and 95% Flex) 
> Hematoxylin- 2 mins
> Rinsing in water until the water runs clear
> Clarifier II- 30 dips
> running water -30 dips
> Richard Allan Bluing - 30 dips
> running water - 30 dips  
> 95% Flex - 10 dips
> Eosin-Y- 5 quick dips
> Thru 95%, 100% etc to xylene
> 
> Any suggestions on a possible method change or a
> more mild Eosin.
> Complaint seems to solely "TOO PINK".  I have done
> some experimenting w/
> cutting the eosin w/ 80% Flex- and it does calm it
> down a bit but, the
> Hemo looks more purply then blue, almost periwinkle
> so not sure if that
> will be better or not- we still need to have him
> review the test slides.
> Any suggestions ?
> 
> Helayne Parker
> Histology Section Head
> Skaggs Community Health Center
> Branson, Missouri
> 
> 
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> End of Histonet Digest, Vol 26, Issue 14
> ****************************************
> 


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