Yaskovich, Ruth A (NIH/NIDCR) [E]
ryaskovich <@t> dir.nidcr.nih.gov
Thu Jan 26 12:17:50 CST 2006
That's from the Armed Forces Institute of Pathology Laboratory Methods in Histotechnology.
National Institutes of Health
National Institute of Dental and Crainiofacial Research
Pain and Neurosensory Mechanism Branch
> From: Gayle Callis
> Sent: Thursday, January 26, 2006 12:55 PM
> To: Ian Montgomery; Histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Advice.
> For some reason, I have a chapter copied from a book titled Animal and
> Insect Histotechnology, no author - all I have is a chapter from this book
> authored by Debra A. McElroy - but it contained some words of wisdom for
> Fixation NBF, Bouins, or Zenkers - after fixation, soak fixed specimens in
> 4% phenol (4 ml melted phenol in 96 ml 80% ethanol) for 24 hours to soften
> the chitnous exoskeletaons then process into paraffin.
> If you try this, please keep us posted on results.
> At 08:29 AM 1/26/2006, you wrote:
> > With cuts in staff I've been asked if I can teach the histology
> > portion of a zoology class. Problem, one of the tissue I'll be using is
> > insect abdomen with its chitin exoskeleton. Any hints and tips regarding
> > this type of material, processing, embedding, sectioning, that sort of
> > thing. Or, is it Mollifix time again?
> >Dr. Ian Montgomery,
> >IBLS Support Services,
> >Graham Kerr Building,
> >Institute of Biomedical & Life Sciences,
> >University of Glasgow,
> >G12 8QQ.
> >Tel: 0141 339 8855
> >Office: 4652
> >Lab: 6644.
> >Pager: 07623 975451
> >e-mail: ian.montgomery <@t> bio.gla.ac.uk
> >Histonet mailing list
> >Histonet <@t> lists.utsouthwestern.edu
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)
> Histonet mailing list
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