[Histonet] Advice.

[Yaskovich, Ruth A (NIH/NIDCR) [E]]

Gayle,
That's from the Armed Forces Institute of Pathology Laboratory Methods in Histotechnology. 
Ruth Yaskovich
National Institutes of Health 
National Institute of Dental and Crainiofacial Research
Pain and Neurosensory Mechanism Branch
Drug Discovery

> ----------
> From: 	Gayle Callis
> Sent: 	Thursday, January 26, 2006 12:55 PM
> To: 	Ian Montgomery; Histonet <@t> lists.utsouthwestern.edu
> Subject: 	Re: [Histonet] Advice.
> 
> For some reason, I have a chapter copied from a book titled Animal and 
> Insect Histotechnology, no author - all I have is a chapter from this book 
> authored by Debra A. McElroy - but it contained some words of wisdom for 
> insects.
> 
> Fixation NBF, Bouins, or Zenkers - after fixation, soak fixed specimens in 
> 4% phenol (4 ml melted phenol in 96 ml 80% ethanol) for 24 hours to soften 
> the chitnous exoskeletaons then process into paraffin.
> 
> If you try this, please keep us posted on results.
> 
> 
> 
>   At 08:29 AM 1/26/2006, you wrote:
> >         With cuts in staff I've been asked if I can teach the histology 
> > portion of a zoology class. Problem, one of the tissue I'll be using is 
> > insect abdomen with its chitin exoskeleton. Any hints and tips regarding 
> > this type of material, processing, embedding, sectioning, that sort of 
> > thing. Or, is it Mollifix time again?
> >
> >Dr. Ian Montgomery,
> >Histotechnology,
> >IBLS Support Services,
> >Graham Kerr Building,
> >Institute of Biomedical & Life Sciences,
> >University of Glasgow,
> >Glasgow,
> >G12 8QQ.
> >Tel: 0141 339 8855
> >Office: 4652
> >Lab: 6644.
> >Pager: 07623 975451
> >e-mail: ian.montgomery <@t> bio.gla.ac.uk
> >
> >
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> 
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)
> 
> 
> 
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>