[Histonet] RE: Microtome configuration
Charles Scouten
cwscouten <@t> myneurolab.com
Thu Jan 26 10:31:38 CST 2006
Sorry, last send had wrong subject line
Cordially,
Charles W. Scouten, Ph.D.
myNeuroLab.com
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300 x 342
FAX 314 522 0377
cwscouten <@t> myneurolab.com
http://www.myneurolab.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Charles
Scouten
Sent: Thursday, January 26, 2006 10:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] skeletal staining
A customer has asked the following question. Can anybody help?
"Can you recommend on what corner I need establish a knife when I'm
working with Vibratome 8000, if I work with Super Mega Cassette and
using Feather mikrotome blade S35".
Cordially,
Charles W. Scouten, Ph.D.
myNeuroLab.com
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300 x 342
FAX 314 522 0377
cwscouten <@t> myneurolab.com
http://www.myneurolab.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rittman,
Barry R
Sent: Thursday, January 26, 2006 9:48 AM
To: Boyce, Amanda (NIH/NIAMS) [F]; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] skeletal staining
No problem
Just make sure that you remove digestive tract as it tends to bind
alizarin.
Barry
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Boyce,
Amanda (NIH/NIAMS) [F]
Sent: Thu 1/26/2006 9:01 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] skeletal staining
I'm in the middle of doing alcian blue/alizarin red whole skeletal chick
preps and I have a question. The protocol I've chosen uses 95% ethanol
for the fixative. I've already fixed in PFA. Is it possible to continue
with the protocol? Are there any steps I should add? Thanks, Amanda
Boyce
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