[Histonet] skeletal staining

[Charles Scouten]

 A customer has asked the following question.  Can anybody help?
 
"Can you recommend on what corner I need establish a knife when I'm
working with Vibratome 8000, if I work with Super Mega Cassette and
using Feather mikrotome blade S35".


Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300 x 342
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
http://www.myneurolab.com 
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rittman,
Barry R
Sent: Thursday, January 26, 2006 9:48 AM
To: Boyce, Amanda (NIH/NIAMS) [F]; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] skeletal staining

No problem
Just make sure that you remove digestive tract as it tends to bind
alizarin.
Barry

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Boyce,
Amanda (NIH/NIAMS) [F]
Sent: Thu 1/26/2006 9:01 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] skeletal staining



I'm in the middle of doing alcian blue/alizarin red whole skeletal chick
preps and I have a question. The protocol I've chosen uses 95% ethanol
for the fixative. I've already fixed in PFA.  Is it possible to continue
with the protocol?  Are there any steps I should add?  Thanks, Amanda
Boyce


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