[Histonet] Qu:Assesment of embryonic BBB defect

[John A. Kiernan]

Immunostain for a plasma protein such as albumin.
It's important to fix the tissue quickly, or
plasma will move out of the blood vessels. See
Mori et al (1991) Lab. Invest. 64:345-351; Fabian
(1992) J. Histochem. Cytochem. 40: 987-991; Loberg
& Torvik (1992) Acta path. microbiol. scand. 100:
431-436. I've seen this artifact in human brain,
with immunostaining for albumin, ceruloplasmin,
orosomucoid and IgG (unpublished). The time of
first formation of the BBB is still controversial
(far too much literature to mention here) and may
depend on the techniques used.
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
Ali Moussavi Nik wrote:
> 
> Dear All
> 
> Hi
> 
> Dose any one knows how to asses the embryonic Blood Brain Barrier defect.
> 
> I would like to see whether or not the Blood Brain Barrier of 18.5 mouse
> embryo of a nock out strain is leaky.
> 
> I will extremely thankful if someone gives me a practical protocol.
> 
> 
> 
> 
> 
> 
> 
> ----------------------------------------------------------------------------
> ------
> 
> Seyed Ali Moussavi Nik D.V.M , PhD Student
> 
> Department of Cell and Molecular Biology,
> 
> Gothenburg University
> 
> Box 462
> 
> S-405 30 Göteborg
> 
> Sweden
> 
> Phone: +46(031) 7733895
> 
> Fax:     +46(031) 7733801
> 
> www.molbio.gu.se
> 
> 
> 
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