[Histonet] (no subject)
kmerriam2003 <@t> yahoo.com
Wed Jan 25 08:14:06 CST 2006
I have used methacarn quite a bit in the past and we did occasionally get a precipitate. When this happened, we would just filter the solution and then use it. It should be fine if you filter the solution. Remember that it should be kept in the fridge and has a limited shelf life, maybe 2 weeks or so in the fridge.
kjsavage <@t> buffalo.edu wrote:
I am having a difficult time trying to make methacarn fixative. I have
searched the list archives and found a posting in May 2005 from someone
that was having the same problem I am facing: a white precipitate
forming. In one of the responses to his posting it was suggested to
use glass only (pipets, vials etc...). I have done that, and still the
precipitate forms. I have also used all new reagents (chloroform,
methanol, acetic acid) and still the precipitate forms. Does anyone
have some more suggestions as to what is going wrong? Thanks, Kathy
Kathy Savage, PhD
Dept. of Exercise and Nutrition Sciences
SUNY-University at Buffalo
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