[Histonet] Perls' Prussian Iron Stain

Paul Bradbury histology.bc <@t> shaw.ca
Tue Jan 24 17:55:34 CST 2006

> Without seeing the brown pigment it is hard to say what it is. But, if 
> you are dealing with sections of mouse liver, my first guess would be 
> that the brown material is bile pigment. You are correct in assuming 
> that the blue material is the product formed by the reaction of ferric 
> iron with Perls' reagents.

You say that you see a big difference between treated and untreated 
animals. What sort of treatment have they had?  Let us have some more 
information, and maybe we can help some more.

Kamloops, BC


>  I did Perls' stain on mouse liver. I undersstand that the bright blue is the target I should focus on. But I also see a lot dark brown stain on them which I don't know what it is. I am eager to know because I do see big difference between treated and untreated animals. I checked my text book and other resouces but could not find the answer. I hope the histonetters here can kindly help me with this.
>  Thanks!
>  Wen
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