[Histonet] pas
Smith, Jeffery D. (HSC)
Jeffery-Smith <@t> ouhsc.edu
Tue Jan 24 16:28:23 CST 2006
Stock Light Green
Light Green 1gm
Distilled water 500 mL
Glacial Acetic Acid 1mL
Mix well and filter. Stock solution stable for 3 to 4 months.
Working Light Green
Stock light green 10mL
Distilled Water 50mL
________________________________
From: BennettW <@t> pac.dfo-mpo.gc.ca [mailto:BennettW <@t> pac.dfo-mpo.gc.ca]
Sent: Tue 1/24/2006 3:55 PM
To: Smith, Jeffery D. (HSC)
Subject: RE: [Histonet] pas
Hi Diane,
What concentration of Light Green did you use?
Cheers
Bill Bennett
Histologist
Fisheries and Oceans Canada
Pacific Biological Station
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Smith,
Jeffery D. (HSC)
Sent: Tuesday, January 24, 2006 1:18 PM
To: Santana, Diane; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] pas
I run a routine PAS just changing the counterstain from hematoxylin to Light
Green. The green masks most of the other components leaving bright pink
fungi on a pink background.
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Santana, Diane
Sent: Tue 1/24/2006 2:10 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] pas
Hi,
I am just curious on how others are doing a PAS for fungus? Thanks Diane
Santana
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