[Histonet] BrdU Staining HCl pretreat

MVaughan4 <@t> ucok.edu MVaughan4 <@t> ucok.edu
Mon Jan 23 13:57:33 CST 2006

Did you pretreat the sample with 2N or 4N HCl?

Melville B. Vaughan, Ph. D.
Assistant Professor
Department of Biology
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034

I am trying to do a BrdU staining on frozen tissue slices (already fixed 
with PFA 4%) for weeks.
The signal is restricted to the rim of the core and not within it. I am 
staining with anti-BrdU by the Roche detection kit. I already tried their 
protocol but that did not work on my fixed slices.
I dry the slices then rehydrate for 1 h. After that I apply the 1st 
antibody for 30 min 37°C.
Thanks for support.

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