[Histonet] BrdU staining

eva-moeller <@t> arcor.de eva-moeller <@t> arcor.de
Mon Jan 23 05:55:04 CST 2006

Hello everybody,

I am trying to do a BrdU staining on frozen tissue slices (already fixed with PFA 4%) for weeks.
The signal is restricted to the rim of the core and not within it. I am staining with anti-BrdU by the Roche detection kit. I already tried their protocol but that did not work on my fixed slices.
I dry the slices then rehydrate for 1 h. After that I apply the 1st antibody for 30 min 37°C.
Thanks for support.

Machen Sie aus 14 Cent spielend bis zu 100 Euro!
Die neue Gaming-Area von Arcor - über 50 Onlinespiele im Angebot.

More information about the Histonet mailing list