[Histonet] BrdU staining

[eva-moeller <@t> arcor.de]

Hello everybody,

I am trying to do a BrdU staining on frozen tissue slices (already fixed with PFA 4%) for weeks.
The signal is restricted to the rim of the core and not within it. I am staining with anti-BrdU by the Roche detection kit. I already tried their protocol but that did not work on my fixed slices.
I dry the slices then rehydrate for 1 h. After that I apply the 1st antibody for 30 min 37°C.
Thanks for support.
Eva

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