[Histonet] Lot to lot antibody validation

Amy Porter portera <@t> msu.edu
Fri Jan 20 08:45:41 CST 2006

We use a known postive control and run a system negative (no primary) with 
each new lot of primary that is received.  We only run it one time maybe at 
the dilution for the old lot then slightly higher and slightly lower 
dilution with the already known pretreatment.  Slides are then reviewed and 
the acceptable dilution for the new lot is recorded on an "Antibody 
Titration Log Sheet" signed off on by the pathologist or researcher to whom 
the work is being provided for.  Hope this helps out...
----- Original Message ----- 
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, January 20, 2006 9:09 AM
Subject: [Histonet] Lot to lot antibody validation

>I would like to hear what procedures people are using to validate different
> lots of antibodies,  including # of specimens you might use for the
> validation.
> Thanks, Brett
> Brett M. Connolly, Ph.D.
> Merck & Co., Inc.
> MRL, Imaging Research
> WP-44K
> PO Box 4
> West Point, PA 19486
> PH 215-652-2501
> fax. 215-993-6803
> e-mail. brett_connolly <@t> merck.com
> ------------------------------------------------------------------------------
> Notice:  This e-mail message, together with any attachments, contains 
> information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New 
> Jersey, USA 08889), and/or its affiliates (which may be known outside the 
> United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as 
> Banyu) that may be confidential, proprietary copyrighted and/or legally 
> privileged. It is intended solely for the use of the individual or entity 
> named on this message.  If you are not the intended recipient, and have 
> received this message in error, please notify us immediately by reply 
> e-mail and then delete it from your system.
> ------------------------------------------------------------------------------
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

More information about the Histonet mailing list