[Histonet] acetylcholinesterase staining

[John Kiernan]

The dire warning following your enquiry is
discouraging! Nevertheless, I make bold to reply.

I have worked in the esterase histochemistry
field, especially choline esterases, since the
early 1960s, when most of the principles had been
established. 

Which method is failing to work for you half of
the time? These methods are extremely reliable if
you go by the book (any book). Complete substrate
solutions cannot be kept and used next day; that's
a rule that applies to just about all enzyme
activity histochemistry. 

John Kiernan
Anatomy, UWO
London, Canada
-----------------------
Angela Bitting wrote:
> 
> Our track record here with our acetylcholinesterase stain is about 50/50. We make up all the solutions fresh on Monday and it works fine, by Tuesday ...nothing. Can anyone send me a good reliable Acetylcholinesterase staining method? Maybe someone elses will work better for us.
> 
> IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet