[Histonet] Counter stain - P.S.
Andrea T. Hooper
anh2006 <@t> med.cornell.edu
Thu Jan 19 15:52:54 CST 2006
Good point. I personally use hematoxylin for many of my weak nuclear
stains especially when quantitating the stain. I just stain them very
>P.S. Dyes like light green or fast green, which stain everything the same
>shade and don't differentiate nuclei, are suitable as counterstains for some
>immuno work. However, in staining for a nuclear antigen it is particularly
>important to have the nuclei visible. Otherwise how can you be sure that
>the staining you see is actually in the nuclei? That's the advantage of
>methyl green. It doesn't stain anything very dark, but it does stain the
>nuclei a little darker than the cytoplasm, so you can see where they are.
More information about the Histonet