[Histonet] Counter stain

Andrea T. Hooper anh2006 <@t> med.cornell.edu
Thu Jan 19 12:44:47 CST 2006

"Light Green" from Biomeda (can be ordered through VWR). It is a 
light green cytoplasmic stain, very nice actually!!

>I am doing a peroxidase DAB stain for an antigen that is present 
>only in small quantities in the nucleus. I am doing it on rat bone. 
>If I counterstain with hematoxylin it covers the DAB stain. What 
>else can I use that will only stain the cytoplasm and not the 
>nucleus. i thought of methyl green but it too is supposed to stain 
>the nucleus. I thought of neutral red but it won't look so good with 
>the brown. Suggestions?
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