Fw: [Histonet] Invertebrates in Sediment

[Bryan Hewlett]

----- Original Message ----- 
From: "Bryan Hewlett" <bhewlett <@t> cogeco.ca>
To: <BennettW <@t> pac.dfo-mpo.gc.ca>
Sent: Thursday, January 19, 2006 1:14 PM
Subject: Re: [Histonet] Invertebrates in Sediment


> Bill,
>
> The third edition of "Staining Procedures used by the Biological Stain 
> Commission" edited by George Clark,
> gives a procedure (Walton 1952) for staining foraminiferans alive at the 
> time of fixation of marine sediments "without incidental staining of 
> organic or inorganic debris".
>
> The concentration of Rose Bengal is given as 0.1% aqueous and the staining 
> time as 10 minutes.
> There is a footnote that the concentration of dye is not critical.
> Since the dye is also a fluorochrome, enumeration may be easier at low 
> power by fluorescence microscopy.
>
> Bryan
>
> ----- Original Message ----- 
> From: <BennettW <@t> pac.dfo-mpo.gc.ca>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Thursday, January 19, 2006 12:24 PM
> Subject: [Histonet] Invertebrates in Sediment
>
>
>> Hello Histonetters,
>>
>> Thanks in advance for spending any time with this query!  I have a
>> researcher that has collected sediment samples, preserved with NBF, and
>> wants to stain any invertebrates in the sediment so that they can be
>> enumerated and identified.  The researcher wants to use rose bengal but
>> doesn't know at what concentration and neither do I.  Can anyone help?
>>
>> Thanks
>> Bill Bennett
>> Histologist
>> Fisheries and Oceans Canada
>> Pacific Biological Station
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>