[Histonet] IHC losing tissue
Rene J Buesa
rjbuesa <@t> yahoo.com
Wed Jan 18 14:40:53 CST 2006
You have a very good point there! I have been also told that the (+) slides
after their expiration date can lose their properties. That fact did not come
to my mind becasuse we were never confronted with it since we used all
our (+) slides before the expiration date.
This is something that should be considered also.
MVaughan4 <@t> ucok.edu wrote:
About your IHC slides losing tissues during processing: It's probably what
Rene and Linda said - preheating is necessary. But I have heard that the
treated slides purchased from vendors lose their stickiness over time. You
might check other lots or other vendors to see if you still have problems.
Were these slides brand new? Has anybody else had problems with old
pretreated slides losing their stickiness?
Melville B. Vaughan, Ph. D.
Department of Biology
University of Central Oklahoma
Edmond, OK 73034
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