[Histonet] re: paraformaldehyde crystals v Prills
carl.hobbs <@t> kcl.ac.uk
Wed Jan 18 08:17:08 CST 2006
Thank you for the three replies. Much appreciated. My apologies for any
I regularly use a Formalin fixing solution derived from the concentrated
Formalin solution. I also need to use a Formalin fixing solution derived
from Paraformaldehyde for certain immunostaining instances. I do make up the
latter in bulk and freeze in the small aliquots required for T/C cell
I don't have any problem making up either of the solutions.
The latter dissolves completely.
What I really want to do is to use Prills instead of paraformaldehyde
crystals. Unfortunately, they do not completely dissolve - around 80%
disssolved after 24hrs. Sure , I can filter afterwards, but, I want to
establish the SOP with the least risk/handling . Hence my request for help.
Does anyone have any experience of using Prills?
Thanks for the "Fischer" suggestion....I will check them out over here.
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