[Histonet] Loosing NoveRed signal

Amy Porter portera <@t> msu.edu
Tue Jan 17 15:19:01 CST 2006

Sarka - We use Nova Red in our laboratory on a regular basis and do not 
encounter this problem.  However, I do know if you review the product insert 
it will tell you that if you use Deionized water opposed to glass distilled 
it will inhibit the reaction.  "Deionized water may contain inhibitors of 
the peroxidase reaction".  We rinse our slides in distilled water and 
counterstain with hematoxylin, dehydrate, clear in xylene.  We have even 
left them overnight in xylene to see if the reaction is removed and it has 
not been a problem.  I would think it might be connected to the water 
----- Original Message ----- 
From: "Sarka Lhotak" <lhotaks <@t> mcmaster.ca>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, January 17, 2006 3:18 PM
Subject: [Histonet] Loosing NoveRed signal

> Hello Histonetters,
> this is a very annoying problem that I see intermitently. While
> developing a colour with NovaRed, I can see a nice staining. I stop the
> reaction with distilled water, do several changes in distilled and let
> sit for 10 minutes. Then Hematoxylin, alcohols, xylenes and
> coverslipping in Permount. Once counterstained and coverslipped, the
> staining is gone! THis problem is not very frequent but all the more
> frustrating.
> Once I talked to Vector about this and was advised to let the slides
> sit in water for 10 minutes before hematoxylin. But it does not always
> help. Since the problem is intermittent I still have not figured out
> what is happening. It is very frustrating, you see a great staining but
> then it is gone and nobody will believe you! Has anybody experienced
> this and have a remedy?
> Thanks for your help,
> Sarka Lhotak
> Henderson Research Centre, McMaster University,
> Hamilton, Ontario
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