[Histonet] Loosing NoveRed signal

[Sarka Lhotak]

Hello Histonetters,
this is a very annoying problem that I see intermitently. While
developing a colour with NovaRed, I can see a nice staining. I stop the
reaction with distilled water, do several changes in distilled and let
sit for 10 minutes. Then Hematoxylin, alcohols, xylenes and
coverslipping in Permount. Once counterstained and coverslipped, the
staining is gone! THis problem is not very frequent but all the more
frustrating.
Once I talked to Vector about this and was advised to let the slides
sit in water for 10 minutes before hematoxylin. But it does not always
help. Since the problem is intermittent I still have not figured out
what is happening. It is very frustrating, you see a great staining but
then it is gone and nobody will believe you! Has anybody experienced
this and have a remedy?
Thanks for your help,

Sarka Lhotak

Henderson Research Centre, McMaster University,
Hamilton, Ontario