[Histonet] Cryosectioning mouse Eyeball

[Patrick Laurie]

I have a couple of Researchers here who are interested in cryosectioning
mouse eyeballs to look at the corneas.  I have given them a few hints
(colder temperatures, cutting quickly and evenly, etc.) but they are
having variable results.  I was wondering if anyone has any further
hints.  Is there some sort of freezing media that someone would
recommend?  We are using Tissue-Tek OCT.  Thanks,

Patrick Laurie, HT 
Benaroya Research Institute
1201 9th Ave
Seattle, Wa  98101
(206) 341-0681