[Histonet] Fixation time

Gudrun Lang gu.lang <@t> gmx.at
Tue Jan 17 05:22:41 CST 2006

I have a question to all fixation-experts.
Can anyone tell me exact pentration- and fixation times, or the mm/hour for
4-8% NBF.
I know it depends on temperature, tissue-quality, but I have not found a
table about this.

In "Histologische Technik, H.C. Burck" stands, that NBF takes 16 hours for
4mm in liver. (1mm/h)
In the Microwave-book, Kok and Boon, stands NBF penetrates fast and the
linkages develope slowly. But was is fast and slowly?

In daily work we let the tissue-blocks beetween 4-8 hours in NBF. Biopsies
sometimes only 2 hours or less. 

Is the ratio 1mm/h really the rule of thumb for penetration and fixation?

Thank you

Gudrun Lang

Histolog. Labor
Akh Linz

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