[Histonet] Fixation time
gu.lang <@t> gmx.at
Tue Jan 17 05:22:41 CST 2006
I have a question to all fixation-experts.
Can anyone tell me exact pentration- and fixation times, or the mm/hour for
I know it depends on temperature, tissue-quality, but I have not found a
table about this.
In "Histologische Technik, H.C. Burck" stands, that NBF takes 16 hours for
4mm in liver. (1mm/h)
In the Microwave-book, Kok and Boon, stands NBF penetrates fast and the
linkages develope slowly. But was is fast and slowly?
In daily work we let the tissue-blocks beetween 4-8 hours in NBF. Biopsies
sometimes only 2 hours or less.
Is the ratio 1mm/h really the rule of thumb for penetration and fixation?
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