[Histonet] paraformaldehyde crystals v Prills
carl.hobbs <@t> kcl.ac.uk
Tue Jan 17 03:00:13 CST 2006
Many of our Groups use paraformaldehyde crystals ( Sigma P6148) to make up
their solution of formalin. Works very well; 4g + 1tab PBS + 6microlitre
10MNaOH.....stir overnight. Dissolved completely:pH7.2
I am trying to get away from using this paraformaldehyde: it is very light
and prone to spread around the fume hood when it's weighed out.
So, I bought PRILLS from Sigma:Problem is, they just do not dissolve
completely, unless I preboil the water.( heating to 60C is not hot enough to
dissolve the Prills.
I have tried all combinations of the formulation above( eg: no NaOH,
dissolving PBS tablet before adding paraformaldehyde).
I want the procedure with the least risk; boiling may be a higher risk than
the spread of powder.
NB: I cannot turn down/off the fume hoods, for the weighing of the
crystals( which behave more like a very fine powder), hence my desire to use
the heavier Prills.
Be grateful for any insights.
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