[Histonet] how to distinguish liver sinusoidal endothelial cells from myofbroblast or hepatic stellate cells?

[yining zhsng]

hi
I am a graduate student working on hepatic stellate
cells(HSC).A mesenchymal cells closes to myofibroblast on
phenotype.
I was required recently by a reviewer to mark out the
location of liver sinusoidal endothelial cells (LSEC) on
rat liver tissue to verify that a-SMA positive cells I
showed are perisinusoidal cells.I tried to find out some
markers for LSEC,such as CD31,CD34 and VWF,etc.But the
comment for these markers are controversial.CD31 seems the
best option but some papers mentioned that it is also
expressed by myofibroblast & HSC.Does anyone give me any
advices how can I demonstrate that HSCs,a-SMA positive
cells are persinusoidal cells?
Any information would be help and thanks for your help.
yining
Division of Gastroenterology and Hepatology,
> University of Tsukuba
> 1-1-1	Tennoudai, Tsukuba City, Japan 305-8575




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