[Histonet] cresyl violet

Rene J Buesa rjbuesa <@t> yahoo.com
Tue Jan 10 09:34:16 CST 2006


Hi Mohana:
  For neurons I always preferred Tress & Tress formula.
  Sol.A: dist. water &#8594; 100mL + acetic acid&#8594;0.01 mL + cresyl violet&#8594; 0.5g
  Sol.B: chloroform&#8594;75mL + abs.ethanol&#8594;12.5 mL+ ether&#8594;12.5mL
  Sol.C: hydrochloric acid&#8594;0.003 mL in 100 mL 95% ethanol.
  Sol.D: sodium bicarbonate&#8594; 0.01g + 95% ethanol&#8594; 100 mL
  Protocol:
  dewash-hydrate sections&#8594; Sol.Ax 30 min. at 50ºC &#8594;wash&#8594;to% ethanol until no more blue washes out&#8594; sol.B x 2-5 min.&#8594; sol.C until differentiation is complete&#8594; sol.D x 2-3 min&#8594; wash in 95% ethanol&#8594; dehydrate with acetone&#8594;clear&#8594;cover slip.
  Hope this will help.
  René J.
MOHANA <mohana_g2002 <@t> yahoo.com> wrote:
  hello !!

i am doing a cresyl violet staining to study the neurons in chick hippocampus.....but d staining sometimes does nt cm..... any suggestions why??
n does this stain helps to understaind neuronal morphology ??

mohana



Mohana


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