[Histonet] cresyl violet
Rene J Buesa
rjbuesa <@t> yahoo.com
Tue Jan 10 09:34:16 CST 2006
Hi Mohana:
For neurons I always preferred Tress & Tress formula.
Sol.A: dist. water → 100mL + acetic acid→0.01 mL + cresyl violet→ 0.5g
Sol.B: chloroform→75mL + abs.ethanol→12.5 mL+ ether→12.5mL
Sol.C: hydrochloric acid→0.003 mL in 100 mL 95% ethanol.
Sol.D: sodium bicarbonate→ 0.01g + 95% ethanol→ 100 mL
Protocol:
dewash-hydrate sections→ Sol.Ax 30 min. at 50ºC →wash→to% ethanol until no more blue washes out→ sol.B x 2-5 min.→ sol.C until differentiation is complete→ sol.D x 2-3 min→ wash in 95% ethanol→ dehydrate with acetone→clear→cover slip.
Hope this will help.
René J.
MOHANA <mohana_g2002 <@t> yahoo.com> wrote:
hello !!
i am doing a cresyl violet staining to study the neurons in chick hippocampus.....but d staining sometimes does nt cm..... any suggestions why??
n does this stain helps to understaind neuronal morphology ??
mohana
Mohana
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