[Histonet] 50 micron mouse brain

[James Watson]

Atoska,
I our lab we are able to cut 250 micron paraffin sections of Golgi
stained whole mouse brains.  We add 10% glycerin in our absolute
alcohols on the processor for the Golgi stain to keep the tissue soft
(we use 5% glycerin in our absolute alcohols on all our animal tissue
processing- this shortens our soaking times greatly, eliminates the need
to chill blocks, and eliminates cracking or drying artifact in the
animal tissue). Then we section the blocks on a sliding microtome, with
a long soak of warm water, moving the knife through the block very
slowly.  We also cut 20-50 micron sections on our rotary microtomes of
mouse brains that have been processed with the 5% glycerin, but that
does require long soaking times.  Good luck.

James Watson HT, ASCP
Facilities Manager of Histology
GNF, Genomics Institute of the Novartis Research Foundation
Room C015
858-332-4647
jwatson <@t> gnf.org 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Atoska
S. Gentry
Sent: Thursday, January 05, 2006 2:36 PM
To: Histonet
Subject: [Histonet] 50 micron mouse brain


Hello, will someone please give me pointers on obtaining good 50u
sections 
of PFA drop fixed and/or immersion fixed coronal paraffin sections of
mouse 
brain? As I section they tend to roll up in tight rolls. I've tried 
sectioning at room temp. vs. chilled sections. Any assistance provided
will 
be greatly appreciated. Thanks, Atoska


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