[Histonet] Processing/embedding question - your opinion

[Allen, Rhonda]

Renee,
	It may be your opinion, but to those of us who have done it out of necessity, not being "lazy and indolent", it sounds like you are trying to make us feel "lazy and indolent".

Rhonda Allen BA HT(ASCP)HTL, QIHC
Histotechnology Specialist II
Stowers Institute
1000 E. 50th Street
Kansas City, MO 64110 
816-926-4305



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, January 06, 2006 11:43 AM
To: Bonnie Whitaker; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Processing/embedding question - your opinion


Bonnie:
  That is my personal and very particular opinion, and I think I am entitled to that,
  in the same way that you have expressed yours.
  I believe that this is covered by the freedom of expression, is it not?
  René J.

Bonnie Whitaker <bwhitaker <@t> brownpathology.com> wrote:
  Rene,

What is "lazy and indolent" about saving processed tissue without embedding it? I frequently collect many tissues for embedding in multi-tissue blocks, and I see no point in embedding the tissue temporarily while I continue to collect additional material. It makes no difference if the paraffin coating is minimal or a full block. You can also store a lot more control tissue in a small area if it is maintained in unembedded cassettes. One cassette can hold several blocks worth of material.

Bonnie Whitaker
Lab Manager
Brown & Associates Medical Laboratories
8076 El Rio
Houston, Texas 77054
713-741-6677

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, January 06, 2006 10:58 AM
To: foley1; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Processing/embedding question - your opinion


Hi Foley:
I have seen that done in several places. I have also seen all the cassettes wrapped in aluminum foil and kept that way (like a pizza left-over). I cannot tell you what would be the consequences for the morphology or future tests. Theoretically speaking probably this practice will not affect because the tissue itself and all its components are supposedly embedded in paraffin that will just solidify. I personally do not like this to be done. For me, personally, it indicates laziness, indolence and even "disrespect" for the tissue sample. Once I was confronted with the need of keeping processed cassettes in a secure way before casting the blocks. My solution was to put all the cassettes in a shallow plastic container, place all the blocks in it, add melted paraffin and prepare one single block, as large as the container. When I was able to prepare the blocks individually, I melted the paraffin and prepared the blocks. Hope this will help you! René J.

foley1 wrote:
Does anyone routinely allow for the hot wax to drain off multiple cassettes of processed tissue and be held at room temperature for multiple days (6
days) before embedding? What would the consequences be to morphology, possible immunohistochemistry and molecular (DNA) studies?



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