[Histonet] Processing/embedding question - your opinion

[Rene J Buesa]

Hi Foley:
  I have seen that done in several places. I have also seen all the cassettes wrapped in aluminum foil and kept that way (like a pizza left-over).
  I cannot tell you what would be the consequences for the morphology or future tests.
  Theoretically speaking probably this practice will not affect because the tissue itself and all its components are supposedly embedded in paraffin that will just solidify.
  I personally do not like this to be done. For me, personally, it indicates laziness, indolence and even "disrespect" for the tissue sample.
  Once I was confronted with the need of keeping processed cassettes in a secure way before casting the blocks. My solution was to put all the cassettes in a shallow
  plastic container, place all the blocks in it, add melted paraffin and prepare one single block, as large as the container. When I was able to prepare the blocks individually, I melted the paraffin and prepared the blocks.
  Hope this will help you!
  René J.

foley1 <foley1 <@t> niehs.nih.gov> wrote:
  Does anyone routinely allow for the hot wax to drain off multiple cassettes
of processed tissue and be held at room temperature for multiple days (6
days) before embedding? What would the consequences be to morphology,
possible immunohistochemistry and molecular (DNA) studies?



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