[Histonet] RE: optimal thickness for cutting of IHC sections

[C.M. van der Loos]

   Sorry  guys  my  message yesterday was somewhat distorted. Here it is   again...
   

   Dear Paul,
   

   I totally agree what you wrote about IHC: it's just a surface event.
   
   Long  time  ago  we  had  to  prove  that our (prehistoric) attempt of
   quantify   due  to  microtome   different  thickness  and gue   on  the  staining  intensity.  The  only   thicker  sections  showed  a higher non-specific b   than thinner sections. See:
   

   CM  van  der  Loos, MMH Marijianowski and AE Becker: Quantification in
   immu   collagen types I   

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands
   


   

   Date: Tue, 3 Jan 2006 13:09:20 -0500
   From: "Monfils, Paul" <PMonf   Subject:  RE:  [Histonet]  optimal  thickness  for  cutti   sections
   To: [1]'histonet <@t> lists.utsouthwestern.edu'   I  believe  section thickness is less critical for IHC because antibo   dies are
   very  large  molecules  that  don't  penetrate  tissue very well, so    regardless of
   the  thickness  of  the  section,  you  are  really only staining   exposed
   surface  of  the  tissue, perhaps to a depth of 2 microns or s   have
   verified  this by electron microscopy.  This is qui   standard
   histochemical  procedures  where a thicker sect   intense
   stain  because  the small dye molecules pene   and stain
   it all the way through.
   
   

   
   

   
  
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References

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