[Histonet] Re: CD4 and CD8 in mouse / human FFPE

[Kuo Christy]

Hi Reinhard,
Could I also ask for a copy of your protocol, cuz the results from
anti-CD4(Novocvastra)  seems to be very unspecific on human tissue
FFPE sections and I don't know the reason.

Thank you for your help!

Best wishes,
Christy
christy0424 <@t> gmail.com

On 12/19/05, Laurie Reilly <laurie.reilly <@t> jcu.edu.au> wrote:
> Reinhard,
> Could I please have a copy of your protocol, we have had lots of trouble
> trying to stain with
> CD4 and CD8 in bovine tissues.
> Any advice you can give would be most welcome, particularly about antigen
> retrieval.
>
>       Hope everyone has a happy Christmas,
>              Regards,
>                          Laurie.
>
> At 08:49 PM 18/12/2005 +0100, you wrote:
> >Hi Patsy,
> >we do a lot of CD4 and CD8 in human FFPE tissues, even in BM after
> >decalification.
> >It works very good and is daily routine in our lab. If you need advice,
> >give me a mail.
> >We now got some hints how both markers could work in mouse tissue, too. If
> we
> >succeed, we'll let you know.
> >
> >best regards
> >Reinhard.
> >
> >
> >
> >PD Dr. med. Reinhard von Wasielewski
> >
> >
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> >Histonet <@t> lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> Mr.Laurie Reilly                                              Ph 07 4781
> 4468
> School of Veterinary & Biomedical Science      Fax  07 4779  1526
> James Cook University
> Townsville  Qld.
> 4811                                      laurie.reilly <@t> jcu.edu.au
>
> Australia.
>
>
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