[Histonet] RE: optimal thickness for cutting of IHC sections
C.M. van der Loos
c.m.vanderloos <@t> amc.uva.nl
Thu Jan 5 04:41:48 CST 2006
<BODY><P>Dear Paul,</P> <P>I totally agree what you
wrote about IHC: it's just a <P>Long time ago we had to prove that our (prehistoric) attempt of
qu due to different thi on the staining i that thicker sections staining than thinner sections. style="FONT-SIZE: 10pt; COLOR: black; FONT-FAM Arial"><FONT face=Times><FONT size=3>CM van der Lo os, MMH Marijianowski and AE Becker: Quantification in
immunohistoche collagen types I and I 347-354</FONT></FONT></SPAN>< style="FONT-SIZE: 10pt; COLOR: black; FONT-FAM Arial"><FONT face=Times><FONT size=3>Chris van der Loos, PhD<BR>Dept. of Pathology<BR>Academic Medical Center
M Nether "urn:schemas-microsoft /><o:p></o:p></FONT></FONT> <P><FONT face=Times></FONT> </P>
<P>Date: Tue, 3 Jan 2006 13:09:20 -0500 <BR>From:
"Mo <PMonfils <@t> Lifespan.org><BR>Subject [Histonet] optimal thickness for cutting of IHC sections< BR>To: <A
href="mailto:'histonet <@t> lists.utsouthwestern. edu'">'histonet <@t> lists.utsouthwestern.edu'</A><BR
> because anti penetrate tissue very of the section, you are exposed<BR>surface of the tissue, perhaps microns or so. We have<BR>verified this b microscopy. This is quite different from standard< BR>histochemical procedures where a thicker section results in a
more i the tissue through.<BR><BR></P>
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